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If This Occurs: Do This:

Start up

During your first login attempt, you receive an error message at the bottom of the Main Window indicating a failed server connection or login.

  1. Configure proxy settings for the connection between your site and the CAS Registry BLAST® server.

  2. Click Exit in the Results Set Manager window.

  3. On the Sequence Assistant page, click Launch CAS Registry BLAST to restart.

If this is unsuccessful, contact (or have your administrator contact) the STN Help Desk.

You attempt to use CAS Registry BLAST and either of the following two messages display:

  • SFGalDeltaBridge;createDeltaClient()
    The Java vm was unable to find the application class. Please check the classpath.

  • Microsoft DirectDraw  – You must be running in 256 colors or higher.

Install the appropriate drivers for your machine; if necessary, select 256 colors, and reboot. (Contact CAS, if desired, for specifics.)

Explanation: On some operating systems, CAS Registry BLAST cannot operate on a display that is set to less than 256 colors.

Search process

You receive a message telling you that submitting the current query will put you over your quota.

Delete a sufficient number of queries to bring you below the quota. Until this occurs, no additional queries can be submitted.

An error message states that your search has failed due to excessive CPU consumption.

Contact CAS.

Your search fails because a search server has gone down after a search has begun, but prior to completion.

Re-run the sequence query. The query process will not restart automatically.

As search completes

This message displays:

  • Sequence: This sequence is too long to display completely. You can get a full copy of this sequence data by contacting CAS at 1-800-753-4227.

Explanation: The sequence length exceeded 32,000 characters.

An error message states that your report has been "trimmed" due to size.

If the original search used multiple database subsets, try re-running the search on individual subsets.

Explanation: When a report exceeds a given threshold, all High Scoring Pairs (HSPs) are deleted from sequences at the end of the report. Re-running the search on subsets should result in smaller individual reports (although E values will differ from those in the original search).

How to tell if deletions have occurred:

  • The alignment bar will not display for HSPs that have been trimmed.
  • If any HSPs appear for a sequence, all HSPs are included for that particular sequence and none have been trimmed.


GenBank® is a registered trademark of the U.S. Department of Health and Human Services for the Genetic Sequence Data Bank.

BLAST® is a registered trademark of the National Library of Medicine.

BLAST® reference information provided in whole or in part from the National Center for Biotechnology Information (NCBI), National Library of Medicine, National Institutes of Health.

Unless designated otherwise, all other information Copyright © 1997-2017 by the American Chemical Society. All rights reserved.